CO1 Amplicon Sequence Variants of bulk arthropod samples (preservative ethanol) collected with Malaise traps from the Insect Biome Atlas project in Sweden

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Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Miraldo A, Sundh J, Iwaszkiewicz-Eggebrecht E, Granqvist E, Manoharan L, Łukasik P, Tack A J M, Andersson A F, Roslin T, Ronquist F (2025). CO1 Amplicon Sequence Variants of bulk arthropod samples (preservative ethanol) collected with Malaise traps from the Insect Biome Atlas project in Sweden. Version 1.2. Swedish Museum of Natural History. Occurrence dataset. https://www.gbif.se/ipt/resource?r=iba_co1_ethanol_2019_se&v=1.2

Droits

Les chercheurs doivent respecter la déclaration de droits suivante:

L’éditeur et détenteur des droits de cette ressource est Swedish Museum of Natural History. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 0b296c00-37aa-482d-8d8a-b332e69b2dc7.  Swedish Museum of Natural History publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Sweden.

Mots-clé

Metadata; Observation

Contacts

Couverture géographique

Sweden

Couverture taxonomique

Insects

Couverture temporelle

Données sur le projet

The Insect Biome Atlas (IBA) is a large-scale insect project aimed at charting the insect faunas of Sweden and Madagascar and their associated microbiomes using high-throughput DNA sequencing of Malaise trap and soil samples. The main aim is to describe the insect faunas of these two biologically and geologically very different countries in unprecedented detail. The Swedish insect fauna consists of postglacial immigrants and is one of the best studied in the world. Madagascar is the world’s most important biodiversity hotspot, with a long period of evolution in isolation and where many species are critically endangered if not already extinct. During the field phase in 2019-2020, weekly samples were collected in both countries for a 12-month period. Insects were collected using Malaise traps: 203 traps in Sweden and 50 in Madagascar. Several other types of samples and ecological measurements were also collected at all trap sites to gather a full understanding of the ecological roles of the organisms that comprise the insect biomes and associated microbiomes. In total, IBA collected ca 8,000 insect community samples. The identification of all insects in the material and the organisms they interact with, such as pathogens as well as symbiotic fungi and bacteria, was achieved using metabarcoding and high-throughput DNA sequencing. In Sweden the Malaise traps were managed by over 150 volunteers, which makes this project one of the largest citizen science projects to take place in Scandinavia. The Swedish inventory was implemented in collaboration with Station Linné and the Malagasy inventory in collaboration with Madagascar Biodiversity Centre, both very active entomological research stations.

Les personnes impliquées dans le projet:

Méthodes d'échantillonnage

Malaise trap samples were collected at all sampling locations of the Insect Biome Atlas project in Sweden (203 Malaise traps in total). Arthropods were collected in individually barcoded bottles pre-filled with 400 mL of 95% ethanol. Malaise traps were active between January and December 2019. Samples were collected every week during spring and summer (March/April to September/October depending on latitude) and monthly or bi-weekly in the autumn and winter (October/November to March/April, depending on latitude). This resulted in 4753 malaise trap samples. Please note that the results presented here correspond only to extractions from preservative ethanol of 15 bulk arthropod samples. See 'Malaise trap samples' and 'Preservative ethanol' extraction in https://doi.org/10.1101/2024.10.24.619818.

Description des étapes de la méthode:

1. See 'Malaise trap samples' and 'Preservative ethanol' extraction in https://doi.org/10.1101/2024.10.24.619818.

Citations bibliographiques

1. Miraldo, A., Sundh, J., Iwaszkiewicz-Eggebrecht, E., Buczek, M., Goodsell, R., Johansson, H., Fisher, B. L., Raharinjanahary, D., Rajoelison, E. T., Ranaivo, C., Randrianandrasana, C., Rafanomezantsoa, J.-J., Manoharan, L., Granqvist, E., van Dijk, L. J. A., Alberg, L., Åhlén, D., Aspebo, M., Åström, S., Bellviken, A., Bergman, P.-E., Björklund, S., Björkman, M. P., Deng, J., Desborough, L., Dolff, E., Eliasson, A., Elmquist, H., Emanuelsson, H., Erixon, R., Fahlen, L., Frogner, C., Fürst, P., Grabs, A., Grudd, H., Guasconi, D., Gunnarsson, M., Häggqvist, S., Hed, A., Hörnström, E., Johansson, H., Jönsson, A., Kanerot, S., Karlsson, A., Karlsson, D., Klinth, M., Kraft, T., Lahti, R., Larsson, M., Lernefalk, H., Lestander, Y., Lindholm, L.-T., Lindholm, M., Ljung, U., Ljung, K., Lundberg, J., Lundin, E., Malmenius, M., Marquina, D., Martinelli, J., Mertz, L., Nilsson, J., Patchett, A., Persson, N., Persson, J., Prus-Frankowska, M., Regazzoni, E., Rosander, K.-G., Rydgård, M., Sandblom, C., Skord, J., Stålhandske, T., Svensson, F., Szpryngiel, S., Tajani, K., Tyboni, M., Ugarph, C., Vestermark, L., Vilhelmsson, J., Wahlgren, N., Wass, A., Wetterstrand, P., Łukasik, P., Tack, A. J. M., Andersson, A. F., Roslin, T., & Ronquist, F. (2024). Data of the Insect Biome Atlas: A metabarcoding survey of the terrestrial arthropods of Sweden and Madagascar. bioRxiv. https://doi.org/10.1101/2024.10.24.619818

Métadonnées additionnelles