CO1 Amplicon Sequence Variants of bulk arthropod samples (homogenized) collected with Malaise traps from the Insect Biome Atlas project in Sweden

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Miraldo A, Sundh J, Iwaszkiewicz-Eggebrecht E, Granqvist E, Manoharan L, Łukasik P, Tack A J M, Andersson A F, Roslin T, Ronquist F (2024). CO1 Amplicon Sequence Variants of bulk arthropod samples (homogenized) collected with Malaise traps from the Insect Biome Atlas project in Sweden. Version 1.0. Swedish Museum of Natural History. Metadata dataset. https://www.gbif.se/ipt/resource?r=iba_co1_homogenate_2019_se&v=1.0

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Sweden

Project Data

The Insect Biome Atlas (IBA) is a large-scale insect project aimed at charting the insect faunas of Sweden and Madagascar and their associated microbiomes using high-throughput DNA sequencing of Malaise trap and soil samples. The main aim is to describe the insect faunas of these two biologically and geologically very different countries in unprecedented detail. The Swedish insect fauna consists of postglacial immigrants and is one of the best studied in the world. Madagascar is the world’s most important biodiversity hotspot, with a long period of evolution in isolation and where many species are critically endangered if not already extinct. During the field phase in 2019-2020, weekly samples were collected in both countries for a 12-month period. Insects were collected using Malaise traps: 203 traps in Sweden and 50 in Madagascar. Several other types of samples and ecological measurements were also collected at all trap sites to gather a full understanding of the ecological roles of the organisms that comprise the insect biomes and associated microbiomes. In total, IBA collected ca 8,000 insect community samples. The identification of all insects in the material and the organisms they interact with, such as pathogens as well as symbiotic fungi and bacteria, was achieved using metabarcoding and high-throughput DNA sequencing. In Sweden the Malaise traps were managed by over 150 volunteers, which makes this project one of the largest citizen science projects to take place in Scandinavia. The Swedish inventory was implemented in collaboration with Station Linné and the Malagasy inventory in collaboration with Madagascar Biodiversity Centre, both very active entomological research stations.

The personnel involved in the project:

Sampling Methods

Malaise trap samples were collected at all sampling locations of the Insect Biome Atlas project in Sweden (203 Malaise traps in total). Arthropods were collected in individually barcoded bottles pre-filled with 400 mL of 95% ethanol. Malaise traps were active between January and December 2019. Samples were collected every week during spring and summer (March/April to September/October depending on latitude) and monthly or bi-weekly in the autumn and winter (October/November to March/April, depending on latitude). This resulted in 4753 malaise trap samples. Please note that the results presented here correspond only to the samples that were homogenized, ie, every fourth sample collected at each sampling site in Sweden (n=873). See 'Malaise trap samples' and 'Homogenization' extraction in https://doi.org/10.1101/2024.10.24.619818.

Method step description:

1. See 'Malaise trap samples' and 'Homogenization' extraction in https://doi.org/10.1101/2024.10.24.619818.

Bibliographic Citations

1. Miraldo, A., Sundh, J., Iwaszkiewicz-Eggebrecht, E., Buczek, M., Goodsell, R., Johansson, H., Fisher, B. L., Raharinjanahary, D., Rajoelison, E. T., Ranaivo, C., Randrianandrasana, C., Rafanomezantsoa, J.-J., Manoharan, L., Granqvist, E., van Dijk, L. J. A., Alberg, L., Åhlén, D., Aspebo, M., Åström, S., Bellviken, A., Bergman, P.-E., Björklund, S., Björkman, M. P., Deng, J., Desborough, L., Dolff, E., Eliasson, A., Elmquist, H., Emanuelsson, H., Erixon, R., Fahlen, L., Frogner, C., Fürst, P., Grabs, A., Grudd, H., Guasconi, D., Gunnarsson, M., Häggqvist, S., Hed, A., Hörnström, E., Johansson, H., Jönsson, A., Kanerot, S., Karlsson, A., Karlsson, D., Klinth, M., Kraft, T., Lahti, R., Larsson, M., Lernefalk, H., Lestander, Y., Lindholm, L.-T., Lindholm, M., Ljung, U., Ljung, K., Lundberg, J., Lundin, E., Malmenius, M., Marquina, D., Martinelli, J., Mertz, L., Nilsson, J., Patchett, A., Persson, N., Persson, J., Prus-Frankowska, M., Regazzoni, E., Rosander, K.-G., Rydgård, M., Sandblom, C., Skord, J., Stålhandske, T., Svensson, F., Szpryngiel, S., Tajani, K., Tyboni, M., Ugarph, C., Vestermark, L., Vilhelmsson, J., Wahlgren, N., Wass, A., Wetterstrand, P., Łukasik, P., Tack, A. J. M., Andersson, A. F., Roslin, T., & Ronquist, F. (2024). Data of the Insect Biome Atlas: A metabarcoding survey of the terrestrial arthropods of Sweden and Madagascar. bioRxiv. https://doi.org/10.1101/2024.10.24.619818 https://doi.org/10.1101/2024.10.24.619818

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