CO1 Amplicon Sequence Variants of soil and leaf litter arthropod communities collected at Malaise traps from the Insect Biome Atlas project in Sweden

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Miraldo A, Sundh J, Iwaszkiewicz-Eggebrecht E, Granqvist E, Manoharan L, Łukasik P, Tack A J M, Andersson A F, Roslin T, Ronquist F (2024). CO1 Amplicon Sequence Variants of soil and leaf litter arthropod communities collected at Malaise traps from the Insect Biome Atlas project in Sweden. Version 1.3. Swedish Museum of Natural History. Metadata dataset. https://www.gbif.se/ipt/resource?r=iba_co1_soil_litter_2019_se&v=1.3

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關鍵字

Metadata

聯絡資訊

地理涵蓋範圍

Sweden

計畫資料

The Insect Biome Atlas (IBA) is a large-scale insect project aimed at charting the insect faunas of Sweden and Madagascar and their associated microbiomes using high-throughput DNA sequencing of Malaise trap and soil samples. The main aim is to describe the insect faunas of these two biologically and geologically very different countries in unprecedented detail. The Swedish insect fauna consists of postglacial immigrants and is one of the best studied in the world. Madagascar is the world’s most important biodiversity hotspot, with a long period of evolution in isolation and where many species are critically endangered if not already extinct. During the field phase in 2019-2020, weekly samples were collected in both countries for a 12-month period. Insects were collected using Malaise traps: 203 traps in Sweden and 50 in Madagascar. Several other types of samples and ecological measurements were also collected at all trap sites to gather a full understanding of the ecological roles of the organisms that comprise the insect biomes and associated microbiomes. In total, IBA collected ca 8,000 insect community samples. The identification of all insects in the material and the organisms they interact with, such as pathogens as well as symbiotic fungi and bacteria, was achieved using metabarcoding and high-throughput DNA sequencing. In Sweden the Malaise traps were managed by over 150 volunteers, which makes this project one of the largest citizen science projects to take place in Scandinavia. The Swedish inventory was implemented in collaboration with Station Linné and the Malagasy inventory in collaboration with Madagascar Biodiversity Centre, both very active entomological research stations.

參與計畫的人員:

取樣方法

Soil and leaf litter arthropod communities were sampled during the growing season (26th of June 2019 to 27th of July 2019) at each Malaise trap location of the Insect Biome Atlas project in Sweden. Leaf litter arthropod communities were sampled by collecting a total volume of 1 L of leaf litter from five different sites around each Malaise trap, 2.5 meters away from the nearest extremity of the trap. Soil arthropod communities were sampled using soil cores (5.5 cm diameter) with a depth of 10 cm at exactly the same five sites where litter samples were taken. The five soil core samples from each Malaise trap location were pooled and mixed by hand immediately after collection. See 'Soil and litter samples' in https://doi.org/10.1101/2024.10.24.619818.

方法步驟描述:

1. See 'Soil and litter samples' in https://doi.org/10.1101/2024.10.24.619818.

引用文獻

1. Miraldo, A., Sundh, J., Iwaszkiewicz-Eggebrecht, E., Buczek, M., Goodsell, R., Johansson, H., Fisher, B. L., Raharinjanahary, D., Rajoelison, E. T., Ranaivo, C., Randrianandrasana, C., Rafanomezantsoa, J.-J., Manoharan, L., Granqvist, E., van Dijk, L. J. A., Alberg, L., Åhlén, D., Aspebo, M., Åström, S., Bellviken, A., Bergman, P.-E., Björklund, S., Björkman, M. P., Deng, J., Desborough, L., Dolff, E., Eliasson, A., Elmquist, H., Emanuelsson, H., Erixon, R., Fahlen, L., Frogner, C., Fürst, P., Grabs, A., Grudd, H., Guasconi, D., Gunnarsson, M., Häggqvist, S., Hed, A., Hörnström, E., Johansson, H., Jönsson, A., Kanerot, S., Karlsson, A., Karlsson, D., Klinth, M., Kraft, T., Lahti, R., Larsson, M., Lernefalk, H., Lestander, Y., Lindholm, L.-T., Lindholm, M., Ljung, U., Ljung, K., Lundberg, J., Lundin, E., Malmenius, M., Marquina, D., Martinelli, J., Mertz, L., Nilsson, J., Patchett, A., Persson, N., Persson, J., Prus-Frankowska, M., Regazzoni, E., Rosander, K.-G., Rydgård, M., Sandblom, C., Skord, J., Stålhandske, T., Svensson, F., Szpryngiel, S., Tajani, K., Tyboni, M., Ugarph, C., Vestermark, L., Vilhelmsson, J., Wahlgren, N., Wass, A., Wetterstrand, P., Łukasik, P., Tack, A. J. M., Andersson, A. F., Roslin, T., & Ronquist, F. (2024). Data of the Insect Biome Atlas: A metabarcoding survey of the terrestrial arthropods of Sweden and Madagascar. bioRxiv. https://doi.org/10.1101/2024.10.24.619818

額外的詮釋資料